University of Silesia in Katowice

1. Vitamin B₁₂ and its historical significance; biologically active forms of vitamin B₁₂; adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl)-dependent enzymes and their catalytic functions (1hr);
2. Photochemistry of vitamin B₁₂ derivatives from historical perspective; alkyl vs. non-alkyl cobalamins; base-on vs. base-off conformations; triplet states (1hr);
3. Computational approach; modeling structural and electronic properties of cobalamins in solution and inside enzymatic environment; activation of Co-C bond (1hr);
4. Photochemistry of B₁₂-dependent enzymes such as EAL and GLM or MetH and MetH with mutation; photoreceptor CarH (1hr).

Water and lipid soluble vitamins (vitamins A, B₁, B₂, B₃, B₅, B₆, B₉, B₁₂, C, D, E, H and K) and derived aspects (occurrence, physiological importance, symptoms of insufficiency, toxicity). 

1. Vitamin B₁₂ and its historical significance; biologically active forms of vitamin B₁₂; adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl)-dependent enzymes and their catalytic functions (1hr);
2. Photochemistry of vitamin B₁₂ derivatives from historical perspective; alkyl vs. non-alkyl cobalamins; base-on vs. base-off conformations; triplet states (1hr);
3. Computational approach; modeling structural and electronic properties of cobalamins in solution and inside enzymatic environment; activation of Co-C bond (1hr);
4. Photochemistry of B₁₂-dependent enzymes such as EAL and GLM or MetH and MetH with mutation; photoreceptor CarH (1hr).

1. Role of metals in biology; introduction to electronic structure of transition metals and ligand field theory; structure of metalloproteins (1hr);
2. Protein Data Bank (PDB) as resource for chemical, biochemical, and structural explorations of biomolecules with transition metals; heme-containing metalloproteins, non-heme systems, B₁₂-dependent enzymes (1hr);
3. Introduction to molecular mechanics (MM), Density Functional Theory (DFT) and combined QM/MM approach to elucidate structure, reactivity and spectroscopic properties of metalloproteins (1hr);
4. Reaction pathways; location of transition states (TSs); spectroscopic properties of selected metalloenzymes (1hr).