Subject: „Drivers of polyploid genome evolution: repetitive DNA dynamics and chromosome rearrangements”
Unit of the project realization: Faculty of Natural Sciences; Institute of Biology, Biotechnology and Environmental Protection – University of Silesia in Katowice
Unit realizing the PhD student education: Doctoral School at the University of Silesia in Katowice
The project will focus on rDNA evolution and organization in Amaranthus using molecular, cytogenetic, bioinformatic, and phylogenetic approaches to understand post-polyploidization genome evolution, including sequence variation and chromosomal localization. It also investigates karyotype evolution and chromosome number changes, including chromosome fusions and dysploidy, using oligo-labeling probes, fluorescent in situ hybridisation, and comparative cytogenetic analyses.
Research will be carried out in the frame of the project „Drivers of polyploid genome evolution: repetitive DNA dynamics and chromosome rearrangements” (number of the project 2025/59/B/NZ8/00852), financed from NCN under management of dr hab. Bożena Kolano, prof. UŚ
Scope of Duties
- Plant material cultivation and preparation
- Chromosome number analyses and detection of inter- and intraspecific variation
- DNA isolation and cloning of rDNA sequences
- Preparation of chromosome spreads (including 3D polyacrylamide-based preparations)
- Fluorescence in situ hybridization (FISH) and immunostaining
- Epifluorescence and confocal microscopy analyses, including image processing
- Participation in bioinformatic analyses
- Design and application of oligonucleotide probes for FISH
Participation in data analysis, manuscript preparation, and conference presentations
Duration of the scholarship: 48 months
Scholarship amount:
PLN 4 693,24 gross (before the mid-term evaluation) / PLN 5 546,55 gross (after mid-term evaluation)
Full text of the announcement: Drivers of polyploid genome evolution – PhD call pl
Registration Link: https://irk.us.edu.pl/pl/offer/SD-GRANT-2026/programme/20-S3PRZ_NZ800852/
Documents should be submitted by November 15 to the following e-mail address:: bozena.kolano@us.edu.pl